LOCUS mPB-L3-ERT2.TatRRR-mCherry 8093 bp DNA circular DEFINITION mPB-L3-ERT2.TatRRR-mCherry plasmid, complete sequence. FEATURES Location/Qualifiers promoter 232..819 /note="The cytomegalovirus (CMV) promoter" CDS 953..3751 /note="mPB-L3-ERT2" /note="PMID: 17576687" /product="mouse codon-optimized version of piggyBac transposase (mPB) fused to the modified estrogen receptor ligand-binding domain (ERT2)" polyA_signal 3802..4033 /note="The bovine growth hormone (BGH) polyadenylation signal" promoter 4103..4665 /note="The cytomegalovirus (CMV) promoter" CDS 4760..5512 /note="TatRRR-mCherry" /note="PMID: 21321119" /product="The nuclear localization sequence of the HIV-1 Tat protein (TatRRR) fused to the mCherry" polyA_signal 5596..5827 /note="The bovine growth hormone (BGH) polyadenylation signal" rep_origin complement(6279..6952) /note"=pUC origin" CDS complement(7097..7957) /note="AmpR" /product="ampicillin resistance protein" BASE COUNT 1960 a 2221 c 2184 g 1728 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccgcca ccatgggcag 961 cagcctggac gacgagcaca tcctgagcgc cctgctgcag agcgacgacg agctggtcgg 1021 cgaggacagc gacagcgaga tcagcgacca cgtgagcgag gacgacgtgc agtccgacac 1081 cgaggaggcc ttcatcgacg aggtgcacga ggtgcagcct accagcagcg gctccgagat 1141 cctggacgag cagaacgtga tcgagcagcc cggcagctcc ctggccagca acaggatcct 1201 gaccctgccc cagaggacca tcaggggcaa gaacaagcac tgctggtcca cctccaagag 1261 caccaggcgg agcagggtgt ccgccctgaa catcgtgaga agccagaggg gccccaccag 1321 gatgtgcagg aacatctacg accccctgct gtgcttcaag ctgttcttca ccgacgagat 1381 catcagcgag atcgtgaagt ggaccaacgc cgagatcagc ctgaagaggc gggagagcat 1441 gaccggcgcc accttcaggg acaccaacga ggacgagatc tacgccttct tcggcatcct 1501 ggtgatgacc gccgtgagga aggacaacca catgagcacc gacgacctgt tcgacagatc 1561 cctgagcatg gtgtacgtga gcgtgatgag cagggacaga ttcgacttcc tgatcagatg 1621 cctgaggatg gacgacaaga gcatcaggcc caccctgcgg gagaacgacg tgttcacccc 1681 cgtgagaaag atctgggacc tgttcatcca ccagtgcatc cagaactaca cccctggcgc 1741 ccacctgacc atcgacgagc agctgctggg cttcaggggc aggtgcccct tcaggatgta 1801 tatccccaac aagcccagca agtacggcat caagatcctg atgatgtgcg acagcggcac 1861 caagtacatg atcaacggca tgccctacct gggcaggggc acccagacca acggcgtgcc 1921 cctgggcgag tactacgtga aggagctgtc caagcccgtc cacggcagct gcagaaacat 1981 cacctgcgac aactggttca ccagcatccc cctggccaag aacctgctgc aggagcccta 2041 caagctgacc atcgtgggca ccgtgagaag caacaagaga gagatccccg aggtcctgaa 2101 gaacagcagg tccaggcccg tgggcaccag catgttctgc ttcgacggcc ccctgaccct 2161 ggtgtcctac aagcccaagc ccgccaagat ggtgtacctg ctgtccagct gcgacgagga 2221 cgccagcatc aacgagagca ccggcaagcc ccagatggtg atgtactaca accagaccaa 2281 gggcggcgtg gacaccctgg accagatgtg cagcgtgatg acctgcagca gaaagaccaa 2341 caggtggccc atggccctgc tgtacggcat gatcaacatc gcctgcatca acagcttcat 2401 catctacagc cacaacgtga gcagcaaggg cgagaaggtg cagagccgga aaaagttcat 2461 gcggaacctg tacatgagcc tgacctccag cttcatgagg aagaggctgg aggcccccac 2521 cctgaagaga tacctgaggg acaacatcag caacatcctg cccaacgagg tgcccggcac 2581 cagcgacgac agcaccgagg agcccgtgat gaagaagagg acctactgca cctactgtcc 2641 cagcaagatc agaagaaagg ccaacgccag ctgcaagaag tgtaagaagg tcatctgccg 2701 ggagcacaac atcgacatgt gccagagctg tttcgcggcc gccaagctgg gcggcggcgc 2761 ccccgccgtg ggcggcggcc ccaaggccgc cgataaaggg gccgcatctg ctggagacat 2821 gagagctgcc aacctttggc caagcccgct catgatcaaa cgctctaaga agaacagcct 2881 ggccttgtcc ctgacggccg accagatggt cagtgccttg ttggatgctg agccccccat 2941 actctattcc gagtatgatc ctaccagacc cttcagtgaa gcttcgatga tgggcttact 3001 gaccaacctg gcagacaggg agctggttca catgatcaac tgggcgaaga gggtgccagg 3061 ctttgtggat ttgaccctcc atgatcaggt ccaccttcta gaatgtgcct ggctagagat 3121 cctgatgatt ggtctcgtct ggcgctccat ggagcaccca gtgaagctac tgtttgctcc 3181 taacttgctc ttggacagga accagggaaa atgtgtagag ggcatggtgg agatcttcga 3241 catgctgctg gctacatcat ctcggttccg catgatgaat ctgcagggag aggagtttgt 3301 gtgcctcaaa tctattattt tgcttaattc tggagtgtac acatttctgt ccagcaccct 3361 gaagtctctg gaagagaagg accatatcca ccgagtcctg gacaagatca cagacacttt 3421 gatccacctg atggccaagg caggcctgac cctgcagcag cagcaccagc ggctggccca 3481 gctcctcctc atcctctccc acatcaggca catgagtaac aaaggcatgg agcatctgta 3541 cagcatgaag tgcaagaacg tggtgcccct ctatgacctg ctgctggagg cggcggacgc 3601 ccaccgccta catgcgccca ctagccgtgg aggggcatcc gtggaggaga cggaccaaag 3661 ccacttggcc actgcgggct ctacttcatc gcattccttg caaaagtatt acatcacggg 3721 ggaggcagag ggtttccctg ccacagcttg atgactcgag catgcatcta gagggcccta 3781 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt 3841 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 3901 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 3961 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 4021 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 4081 ctagggggta tccccgctag ctattaatag taatcaatta cggggtcatt agttcatagc 4141 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 4201 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 4261 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 4321 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 4381 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 4441 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 4501 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 4561 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 4621 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga 4681 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gaccctcgct 4741 tggtaccgag ctcggatcca tgtatggcag gaagaagcgg agacagcgac gaagaaagct 4801 tggggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 4861 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 4921 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 4981 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 5041 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 5101 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 5161 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 5221 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 5281 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 5341 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 5401 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 5461 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aaccggaatt 5521 ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc cctattctat 5581 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc 5641 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 5701 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 5761 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 5821 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 5881 ggtatccccc cgcgggtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 5941 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 6001 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 6061 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 6121 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 6181 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 6241 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 6361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 6421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 6481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac 6541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 6601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 6661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 6721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 6781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 6841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 6901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 6961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 7021 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 7081 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 7141 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 7201 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 7261 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 7321 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 7381 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 7441 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 7501 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 7561 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 7621 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 7681 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 7741 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 7801 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 7861 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 7921 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 7981 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 8041 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc //